Metabolomics approach to understand the hepatitis C virus induced hepatocellular carcinoma using LC-ESI-MS/MS

Hepatocellular carcinoma (HCC) is a typical cancer that has region specified analysis with the incidence of hepatitis C virus (HCV) infection. This study was conducted to improve understanding metabolic alterations associated HCV induced HCC which can open up new strategies monitor high risk HCC. Samples subjects HCV, chronic liver disease (CLD), HCC, and healthy controls (HS) were collected after complete blood count (CBC), viral load, ?-fetoprotein (AFP), function tests, albumin. A total 147 serum samples including (n = 11), CLD 24), 71), HS 41) analyzed by LC-ESI-MS/MS. The 21 compounds found be responsible for group discrimination application chemometric tools. N-fructosyl tyrosine hydroxyindoleacetic acid showed an increase in level whereas L-aspartyl-L-phenylalanine thyroxine consistent decrease progression comparison indicating their importance early detection. biological pathways such as glycerophospholipid metabolism, phenylalanine, tyrosine, tryptophan biosynthesis, phenylalanine metabolism alteration some metabolites. method internally validated ROC plot showing AUC value HS, CLD, 0.99, 1, 0.89, respectively; while 16 blind also 93% specificity. untargeted metabolomics investigation help understand HCV-induced It reveals significant differences metabolites predict prognostic diagnostic markers.